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Person Details

Superfund Research Program

Sylvia Daunert

University of Miami
University of Miami
1011 NW 15 St.
Gautier Bldg, Room 116
Miami, FL 33136
Phone: 305-243-4005
Fax: 305-243-3955


Research Briefs






  • Hamorsky KT, Dikici E, Ensor CM, Daunert S. 2011. Designer Bioluminescent Systems in Biotechnology. Chapter 13 in: Chemiluminescence and Bioluminescence: Past, Present and Future. Royal Society of Chemistry, Cambridge, United Kingdom.
  • Knecht LD, Pasini P, Daunert S. 2011. Bacterial spores as platforms for bioanalytical and biomedical applications. Anal Bioanal Chem400(4):977-989. doi:10.1007/s00216-011-4835-4
  • Lewis S, Datta S, Gui M, Coker EL, Huggins F, Daunert S, Bachas LG, Bhattacharyya D. 2011. Reactive nanostructured membranes for water purification. Proceedings of the National Academy of Sciences of the United States of America (USA)108(21):8577-8582. doi:10.1073/pnas.1101144108
  • Sangal A, Pasini P, Daunert S. 2011. Stability of spore-based biosensing systems under extreme conditions. Sensors and Actuators B: Chemical158:377-382. doi:10.1016/j.snb.2011.06.039
  • Turner K, Raut N, Pasini P, Daunert S, Michelini E, Cevenini L, Mezzanotte L, Roda A. 2011. Cell-Based Bioluminescent Biosensors. Chapter 15 in: Chemiluminescence and Bioluminescence: Past, Present and Future. Royal Society of Chemistry, Cambridge, United Kingdom. pp.514-545.


  • Date A, Pasini P, Daunert S. 2010. Fluorescent and bioluminescent cell-based sensors: strategies for their preservation. Adv Biochem Eng Biotechnol117:57-75.
  • Date A, Pasini P, Daunert S. 2010. Integration of spore-based genetically engineered whole-cell sensing systems into portable centrifugal microfluidic platforms. Anal Bioanal Chem398(1):349-356. doi:10.1007/s00216-010-3930-2
  • Date A, Pasini P, Sangal A, Daunert S. 2010. Packaging Sensing Cells in Spores for Long-Term Preservation of Sensors: A Tool for Biomedical and Environmental Analysis. Anal Chem82(14):6098-6103. doi:10.1021/ac1007865
  • Struss A, Pasini P, Daunert S. 2010. Biosensing Systems Based on Genetically Engineered Whole Cells. Chapter 14 in: Recognition Receptors in Biosensors. Springer, New York, NY. pp.565-598.


  • Rowe L, Dikici E, Daunert S. 2009. Engineering Bioluminescent Proteins: Expanding their Analytical Potential. Anal Chem81:8662-8668. doi:10.1021/ac9007286


  • Daunert S. 2006. Analytical and bioanalytical chemistry in white waters: 2006 and beyond. Anal Bioanal Chem384(1):1-3.
  • Feliciano JS, Liu Y, Daunert S. 2006. Novel reporter gene in a fluorescent-based whole cell sensing system. Biotechnol Bioeng93(5):989-997.
  • Feliciano JS, Pasini P, Deo SK, Daunert S. 2006. Photoproteins as reporters in whole cell sensing systems. Chapter 8 in: Photoproteins in Bioanalysis. Wiley-VCH, New York, NY. pp.131-154.
  • Feliciano JS, Xu S, Guan X, Lehmler H, Bachas LG, Daunert S. 2006. ClcR-based biosensing system in the detection of cis-dihydroxylated (chloro-)biphenyls. Anal Bioanal Chem385(5):807-813.


  • Rothert A, Deo SK, Millner L, Puckett LG, Madou MJ, Daunert S. 2005. Whole-cell-reporter-gene-based biosensing systems on a compact disk microfluidics platform. Anal Biochem342(1):11-19.
  • Xu S, D'Angelo E, Ghosh D, Feliciano JS, Deo SK, Daunert S. 2005. Detection of polychlorinated biphenyls employing chemical dechlorination followed by biphenyl whole cell sensing system. Toxicological & Environmental Chemistry87(3):287-298. doi:10.1080/02772240400029769


  • Feliciano JS, Guan X, Liu Y, D'Angelo E, van der Meer JR, Bachas LG, Daunert S. 2003. Bacterial sensing systems for monitoring superfund chemicals. In: Proceedings of the NAFF (North American Freedom Foundation) Symposium, 2003. Lexington, KY.
  • Feliciano JS, Guan X, van der Meer JR, Bachas LG, Daunert S. 2003. Bacterial Biosensing Systems for Monitoring EnvironmentalPollutants:From The Laboratory to the Field. In: Proceedings of the International Symposium on Sensor Science, 2003. Paris, France.
  • Feliciano JS, Rothert A, Deo SK, Puckett LG, Millner L, van der Meer JR, Madou MJ, Daunert S. 2003. Bacterial biosensing systems for arsenic detection: from the laboratory to the field. In: Proceedings of the Superfund Basic Research Program Annual Meeting: Integration Perspectives, November 9-12, 2003. Dartmouth College, Hanover, NH.
  • Rothert S, Deo SK, Puckett LG, Millner L, Madou MJ, Daunert S. 2003. Adaptation of a whole-cell based reporter gene assay for arsenite and antimonite to a compact disc centrifugal microfluidics patform. In: Proceedings of the 55th Southeast Regional Meeting of the American Chemical Society (SERMACS), November 16-19, 2003. Atlanta, GA.
  • Stocker J, Balluch D, Gsell M, Harms H, Feliciano JS, Daunert S, Malik KA, van der Meer JR. 2003. Development of a set of simple bacterial biosensors for quantitative and rapid measurements of arsenite and arsenate in potable water. Environ Sci Technol37(20):4743-4750.
  • Xu S, Ghosh A, Feliciano JS, Deo SK, D'Angelo E, Daunert S. 2003. Development of a whole-cell sensing system for PCBs detection. In: Proceedings of the Superfund Basic Research Program Annual Meeting: Integration Perspectives, November 9-12, 2003. Dartmouth College, Hanover, NH.


  • Guan X, D'Angelo E, Luo W, Daunert S. 2002. Whole-cell biosensing of 3-chlorocatechol in liquids and soils. Anal Bioanal Chem374(5):841-847.


  • Butterfield DA, Bhattacharyya D, Daunert S, Bachas LG. 2001. Catalytic biofunctional membranes containing site-specifically immobilized enzyme arrays: a review. Journal of Membrane Science181(1):29-37.
  • Ramanathan S, Shetty RS, Daunert S. 2001. Genetically Engineered Microorganisms as Biosensors for Environmental Toxins. In: Proceedings of the 9th European Congress on Biotechnology.


  • Daunert S, Barrett G, Feliciano JS, Shetty RS, Shrestha S, Smith-Spencer W. 2000. Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes. Chem Rev100(7):2705-2738.
  • Guan X, Ramanathan S, Garris JP, Shetty RS, Ensor CM, Bachas LG, Daunert S. 2000. Chlorocatechol detection based on a clc operon/reporter gene system. Anal Chem72(11):2423-2427.


  • Ramanathan S, Liu Y, Shetty RS, Guan X, Daunert S. 1999. Luminescence-Based Sensing Systems Employing Genetically Engineered Bacteria. In: Proceedings of the 10th International Symposium on Bioluminescence and Chemiluminescence. John Wiley & Sons, Chichester, England. pp.601-604.


  • Bachas LG, Daunert S, Robertson LW. 1998. Information dissemination and outreach activities at the University of Kentucky. In: Proceedings of the Superfund Basic Research Program (SBRP) Conference, 1998. Boston, MA. pp.69.
  • Hernandez EC, Daunert S. 1998. Green Fluorescent Protein as a Novel Label in Binding Assays. Anal Biochem261(1):113-115.
  • Lewis JC, Feltus A, Ensor CM, Ramanathan S, Daunert S. 1998. Applications of reporter genes in analytical chemistry. Anal Chem70(17):579A-585A. doi:10.1021/ac9819638
  • Ramanathan S, Lewis JC, Kindy MS, Daunert S. 1998. Heterogeneous Binding Assay for an Octapeptide Based on a Fusion Protein with Recombinant Aequorin. Anal Chim Acta369:181-188.
  • Ramanathan S, Shi W, Rosen BP, Daunert S. 1998. Bacteria-Based Chemiluminescence Sensing System Using ±-Galactosidase Under the Control of the ArsR Regulatory Protein of the ars Operon. Anal Chim Acta369:189-195.
  • Salins LL, Schauer-Vukasinovic V, Daunert S. 1998. Optical Sensing Systems Based on Biomolecular Recognition of Recombinant Proteins. SPIE Proceedings, San Jose, CA 3270:16-24.


  • Blair TL, Daunert S, Bachas LG. 1997. Host-Guest Chemistryin Fiber Optic Sensing. In: Biosensors, Current Topics in Biophysics.
  • Crofcheck CL, Grosvenor AL, Anderson KW, Lumpp JK, Scott DL, Daunert S. 1997. Detecting Biomolecules in pL-Vials Using the Bioluminescence of Aequorin. Anal Chem69(23):4768-4772.
  • Ensor CM, Ramanathan S, Scott DL, Daunert S. 1997. Engineered Bacteria Can Detect Toxic Metals. Waterworks 3:1-4.
  • Feltus A, Ramanathan S, Daunert S. 1997. Cooperative Interaction of Immobilized Avidin with Aequorin-Biotin Conjugates: An Aequorin-Linked Assay for Biotin. Anal Biochem254:62-68.
  • Grosvenor AL, Crofcheck CL, Anderson KW, Scott DL, Daunert S. 1997. Calibration of Micropipets Using the Bioluminescent Protein Aequorin. Anal Chem69:3115-3118.
  • Lizano S, Ramanathan S, Feltus A, Witkowski A, Daunert S. 1997. Bioluminescence Competitive Binding Assays for Biotin Based on the Photoprotein Aequorin. Methods Enzymol279:296-303.
  • Palet C, Muñoz M, Valiente M, Cynkowski T, Daunert S, Bachas LG. 1997. Molecular Recognition of Dicarboxylic Acids by Neutral Receptors: Selective Membrane Transport. Anal Chim Acta343(3):287-294.
  • Ramanathan S, Ensor CM, Daunert S. 1997. Metal-resistance bacterial systems in sensors. Trends Biotechnol15(12):500-506.
  • Ramanathan S, Shi W, Rosen BP, Daunert S. 1997. Bioluminescence Sensing System For Antimonite with Subattomole Detection Based on Luciferase Reporter Genes. Anal Chem69:3380-3384.
  • Schauer-Vukasinovic V, Cullen L, Daunert S. 1997. Rational Design of a Calcium Sensing System Based on Induced Conformational Changes of Calmodulin. J Am Chem Soc119:11102-11103.
  • Scott DL, Ramanathan S, Shi W, Rosen BP, Daunert S. 1997. Electrochemical-Based Sensing System for Antimonite and Arsenite Using Genetically Engineered Bacteria. Anal Chem69:16-20.


  • Hentz NG, Daunert S. 1996. Bifunctional Fusion Proteins of Calmodulin and Protein A as Affinity Ligands in Protein Purification and in the Study of Protein-Protein Interactions. Anal Chem68(22):3939-3944.
  • Hentz NG, Vukasinovic V, Daunert S. 1996. Affinity Chromatography for Recombinant Peptides/Proteins Based on a Calmodulin Fusion Tail. Anal Chem68(9):1550-1555.
  • Hentz NG, Vukasinovic V, Diez S, Valiente M, Daunert S. 1996. Affinity Chromatography for Recombinant Proteins. In: Biofunctional Membranes (The Language of Science). Plenum Press, pp.73-81.
  • Huang W, Feltus A, Witkowski A, Daunert S. 1996. Homogeneous Bioluminescence Competitive Binding Assay for Folate Based on a Coupled Glucose-6-phosphate Dehydrogenase-Bacterial Luciferase System. Anal Chem68(9):1646-1650.


  • Allen JR, Florido A, Young SD, Daunert S, Bachas LG. 1995. Nitrite-Selective Electrode Based on an Electropolymerized Cobalt Phthalocyanine. Electroanalysis7(8):710-713.
  • Daunert S, Bachas LG, Smith-Palmer T. 1995. Gas Sensing Probes. In: Encyclopedia of Analytical Science. Academic Press, pp.4118-4124.
  • Diez S, Zhou P, Daunert S, Valiente M. 1995. Immobilized Soft-Metal Affinity System for Amino Acids Based on an 8-Hydroxyquinoline-Pd(II) Complex: Characterization Using Glycine as a Model. Anal Chim Acta315:339-345.
  • Hernandez EC, Witkowski A, Daunert S, Bachas LG. 1995. Potentiometric Enzyme Electrodes for Urea Based on Electrochemically Prepared Polypyrrole Membranes. Microchimica Acta121:63-72.
  • Witkowski A, Daunert S, Kindy MS, Bachas LG. 1995. Preparation of β-Galactosidase Conjugates for Competitive Binding Assays by Post-Translational Modification of Recombinant Proteins. Anal Chem67:1301-1306.

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