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Person Details: Sylvia Daunert

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Superfund Research Program

Sylvia Daunert

University of Miami
University of Miami
1011 NW 15 St.
Gautier Bldg, Room 116
Miami, Florida 33136
Phone: 305-243-4005
Fax: 305-243-3955


Research Briefs






  • Hamorsky KT, Dikici E, Ensor CM, Daunert S. 2011. Designer Bioluminescent Systems in Biotechnology. Chapter 13 in: Chemiluminescence and Bioluminescence: Past, Present and Future. Royal Society of Chemistry, Cambridge, United Kingdom.
  • Knecht LD, Pasini P, Daunert S. 2011. Bacterial spores as platforms for bioanalytical and biomedical applications. Anal Bioanal Chem 400(4):977-989. doi:10.1007/s00216-011-4835-4 PMID:21380604
  • Lewis S, Datta S, Gui M, Coker EL, Huggins F, Daunert S, Bachas LG, Bhattacharyya D. 2011. Reactive nanostructured membranes for water purification. Proc Natl Acad Sci U S A 108(21):8577-8582. doi:10.1073/pnas.1101144108 PMID:21606340 PMCID:PMC3102394
  • Sangal A, Pasini P, Daunert S. 2011. Stability of spore-based biosensing systems under extreme conditions. Sens Actuators B Chem 158:377-382. doi:10.1016/j.snb.2011.06.039
  • Turner K, Raut N, Pasini P, Daunert S, Michelini E, Cevenini L, Mezzanotte L, Roda A. 2011. Cell-Based Bioluminescent Biosensors. Chapter 15 in: Chemiluminescence and Bioluminescence: Past, Present and Future. Royal Society of Chemistry, Cambridge, United Kingdom. pp.514-545.


  • Date A, Pasini P, Daunert S. 2010. Fluorescent and bioluminescent cell-based sensors: strategies for their preservation. Adv Biochem Eng Biotechnol 117:57-75. doi:10.1007/10_2009_22 PMID:20091290
  • Date A, Pasini P, Daunert S. 2010. Integration of spore-based genetically engineered whole-cell sensing systems into portable centrifugal microfluidic platforms. Anal Bioanal Chem 398(1):349-356. doi:10.1007/s00216-010-3930-2 PMID:20582692
  • Date A, Pasini P, Sangal A, Daunert S. 2010. Packaging sensing cells in spores for long-term preservation of sensors: a tool for biomedical and environmental analysis. Anal Chem 82(14):6098-6103. doi:10.1021/ac1007865 PMID:20560542
  • Struss A, Pasini P, Daunert S. 2010. Biosensing Systems Based on Genetically Engineered Whole Cells. Chapter 14 in: Recognition Receptors in Biosensors. Springer, New York, NY. pp.565-598.



  • Daunert S. 2006. Analytical and bioanalytical chemistry in white waters: 2006 and beyond. Anal Bioanal Chem 384(1):1-3. PMID:16333600
  • Feliciano JS, Liu Y, Daunert S. 2006. Novel reporter gene in a fluorescent-based whole cell sensing system. Biotechnol Bioeng 93(5):989-997. PMID:16489629
  • Feliciano JS, Pasini P, Deo SK, Daunert S. 2006. Photoproteins as reporters in whole cell sensing systems. Chapter 8 in: Photoproteins in Bioanalysis. Wiley-VCH, New York, NY. pp.131-154.
  • Feliciano JS, Xu S, Guan X, Lehmler H, Bachas LG, Daunert S. 2006. ClcR-based biosensing system in the detection of cis-dihydroxylated (chloro-)biphenyls. Anal Bioanal Chem 385(5):807-813. PMID:16791559


  • Rothert A, Deo SK, Millner L, Puckett LG, Madou MJ, Daunert S. 2005. Whole-cell-reporter-gene-based biosensing systems on a compact disk microfluidics platform. Anal Biochem 342(1):11-19. PMID:15958175
  • Xu S, D'Angelo E, Ghosh D, Feliciano JS, Deo SK, Daunert S. 2005. Detection of polychlorinated biphenyls employing chemical dechlorination followed by biphenyl whole cell sensing system. Toxicol Sci 87(3):287-298. doi:10.1080/02772240400029769


  • Feliciano JS, Guan X, Liu Y, D'Angelo E, van der Meer JR, Bachas LG, Daunert S. 2003. Bacterial sensing systems for monitoring superfund chemicals. In: Proceedings of the NAFF (North American Freedom Foundation) Symposium, 2003. Lexington, KY.
  • Feliciano JS, Guan X, van der Meer JR, Bachas LG, Daunert S. 2003. Bacterial Biosensing Systems for Monitoring EnvironmentalPollutants:From The Laboratory to the Field. In: Proceedings of the International Symposium on Sensor Science, 2003. Paris, France.
  • Feliciano JS, Rothert A, Deo SK, Puckett LG, Millner L, van der Meer JR, Madou MJ, Daunert S. 2003. Bacterial biosensing systems for arsenic detection: from the laboratory to the field. In: Proceedings of the Superfund Basic Research Program Annual Meeting: Integration Perspectives, November 9-12, 2003. Dartmouth College, Hanover, NH.
  • Rothert S, Deo SK, Puckett LG, Millner L, Madou MJ, Daunert S. 2003. Adaptation of a whole-cell based reporter gene assay for arsenite and antimonite to a compact disc centrifugal microfluidics patform. In: Proceedings of the 55th Southeast Regional Meeting of the American Chemical Society (SERMACS), November 16-19, 2003. Atlanta, GA.
  • Stocker J, Balluch D, Gsell M, Harms H, Feliciano JS, Daunert S, Malik KA, van der Meer JR. 2003. Development of a set of simple bacterial biosensors for quantitative and rapid measurements of arsenite and arsenate in potable water. Environ Sci Technol 37(20):4743-4750. PMID:14594387
  • Xu S, Ghosh A, Feliciano JS, Deo SK, D'Angelo E, Daunert S. 2003. Development of a whole-cell sensing system for PCBs detection. In: Proceedings of the Superfund Basic Research Program Annual Meeting: Integration Perspectives, November 9-12, 2003. Dartmouth College, Hanover, NH.


  • Guan X, D'Angelo E, Luo W, Daunert S. 2002. Whole-cell biosensing of 3-chlorocatechol in liquids and soils. Anal Bioanal Chem 374(5):841-847. PMID:12434240


  • Butterfield DA, Bhattacharyya D, Daunert S, Bachas LG. 2001. Catalytic biofunctional membranes containing site-specifically immobilized enzyme arrays: a review. J Memb Sci 181(1):29-37.
  • Ramanathan S, Shetty RS, Daunert S. 2001. Genetically Engineered Microorganisms as Biosensors for Environmental Toxins. In: Proceedings of the 9th European Congress on Biotechnology.


  • Daunert S, Barrett G, Feliciano JS, Shetty RS, Shrestha S, Smith-Spencer W. 2000. Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes. Chem Rev 100(7):2705-2738. PMID:11749302
  • Guan X, Ramanathan S, Garris JP, Shetty RS, Ensor CM, Bachas LG, Daunert S. 2000. Chlorocatechol detection based on a clc operon/reporter gene system. Anal Chem 72(11):2423-2427. PMID:10857616


  • Ramanathan S, Liu Y, Shetty RS, Guan X, Daunert S. 1999. Luminescence-Based Sensing Systems Employing Genetically Engineered Bacteria. In: Proceedings of the 10th International Symposium on Bioluminescence and Chemiluminescence. John Wiley & Sons, Chichester, England. pp.601-604.


  • Bachas LG, Daunert S, Robertson LW. 1998. Information dissemination and outreach activities at the University of Kentucky. In: Proceedings of the Superfund Basic Research Program (SBRP) Conference, 1998. Boston, MA. pp.69.
  • Hernandez EC, Daunert S. 1998. Green fluorescent protein as a novel label in binding assays. Anal Biochem 261(1):113-115.
  • Lewis JC, Feltus A, Ensor CM, Ramanathan S, Daunert S. 1998. Applications of reporter genes. Anal Chem 70(17):579A-585A. PMID:9737200
  • Ramanathan S, Lewis JC, Kindy MS, Daunert S. 1998. Heterogeneous binding assay for an octapeptide based on a fusion protein with recombinant aequorin. Anal Chim Acta 369:181-188.
  • Ramanathan S, Shi W, Rosen BP, Daunert S. 1998. Bacteria-based chemiluminescence sensing system using β-galactosidase under the control of the ArsR regulatory protein of the ars operon. Anal Chim Acta 369:189-195.
  • Salins LL, Schauer-Vukasinovic V, Daunert S. 1998. Optical sensing systems based on biomolecular recognition of recombinant proteins. SPIE Proceedings, San Jose, CA 3270:16-24.


  • Blair TL, Daunert S, Bachas LG. 1997. Host-Guest Chemistryin Fiber Optic Sensing. In: Biosensors, Current Topics in Biophysics.
  • Crofcheck CL, Grosvenor AL, Anderson KW, Lumpp JK, Scott DL, Daunert S. 1997. Detecting biomolecules in pL-vials using the bioluminescence of aequorin. Anal Chem 69(23):4768-4772. PMID:9406527
  • Ensor CM, Ramanathan S, Scott DL, Daunert S. 1997. Engineered bacteria can detect toxic metals. Waterworks 3:1-4.
  • Feltus A, Ramanathan S, Daunert S. 1997. Cooperative interaction of immobilized avidin with aequorin-biotin conjugates: an aequorin-linked assay for biotin. Anal Biochem 254:62-68.
  • Grosvenor AL, Crofcheck CL, Anderson KW, Scott DL, Daunert S. 1997. Calibration of micropipets using the bioluminescent protein aequorin. Anal Chem 69:3115-3118. PMID:9253255
  • Lizano S, Ramanathan S, Feltus A, Witkowski A, Daunert S. 1997. Bioluminescence competitive binding assays for biotin based on the photoprotein aequorin. Methods Enzymol 279:296-303. PMID:9211281
  • Palet C, Muñoz M, Valiente M, Cynkowski T, Daunert S, Bachas LG. 1997. Molecular recognition of dicarboxylic acids by neutral receptors: selective membrane transport. Anal Chim Acta 343(3):287-294.
  • Ramanathan S, Ensor CM, Daunert S. 1997. Metal-resistance bacterial systems in sensors. Trends Biotechnol 15(12):500-506.
  • Ramanathan S, Shi W, Rosen BP, Daunert S. 1997. Bioluminescence sensing system for antimonite with subattomole detection based on luciferase reporter genes. Anal Chem 69:3380-3384.
  • Schauer-Vukasinovic V, Cullen L, Daunert S. 1997. Rational design of a calcium sensing system based on induced conformational changes of calmodulin. J Am Chem Soc 119:11102-11103.
  • Scott DL, Ramanathan S, Shi W, Rosen BP, Daunert S. 1997. Electrochemical-based sensing system for antimonite and arsenite using genetically engineered bacteria. Anal Chem 69:16-20.


  • Hentz NG, Daunert S. 1996. Bifunctional fusion proteins of calmodulin and protein A as affinity ligands in protein purification and in the study of protein-protein interactions. Anal Chem 68(22):3939-3944. PMID:8916452
  • Hentz NG, Vukasinovic V, Daunert S. 1996. Affinity chromatography for recombinant peptides/proteins based on a calmodulin fusion tail. Anal Chem 68(9):1550-1555. PMID:8815745
  • Hentz NG, Vukasinovic V, Diez S, Valiente M, Daunert S. 1996. Affinity Chromatography for Recombinant Proteins. In: Biofunctional Membranes (The Language of Science). Plenum Press, pp.73-81.
  • Huang W, Feltus A, Witkowski A, Daunert S. 1996. Homogeneous bioluminescence competitive binding assay for folate based on a coupled glucose-6-phosphate dehydrogenase-bacterial luciferase system. Anal Chem 68(9):1646-1650. PMID:8815749


  • Allen JR, Florido A, Young SD, Daunert S, Bachas LG. 1995. Nitrite-selective electrode based on an electropolymerized cobalt phthalocyanine. Electroanalysis 7(8):710-713.
  • Daunert S, Bachas LG, Smith-Palmer T. 1995. Gas Sensing Probes. In: Encyclopedia of Analytical Science. Academic Press, pp.4118-4124.
  • Diez S, Zhou P, Daunert S, Valiente M. 1995. Immobilized soft-metal affinity system for amino acids based on an 8-hydroxyquinoline-Pd(II) complex: characterization using glycine as a model. Anal Chim Acta 315:339-345.
  • Hernandez EC, Witkowski A, Daunert S, Bachas LG. 1995. Potentiometric enzyme electrodes for urea based on electrochemically prepared polypyrrole membranes. Microchimica Acta 121:63-72.
  • Witkowski A, Daunert S, Kindy MS, Bachas LG. 1995. Preparation of β-galactosidase conjugates for competitive binding assays by post-translational modification of recombinant proteins. Anal Chem 67:1301-1306.
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