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Your Environment. Your Health.

Final Progress Reports: Michigan State University: Kinetic, Ecological and Genetic Factors Affecting Bioaugmentation of Carbon Tetrachloride-Contaminated Sites with Pseudomonas sp. Strain KC

Superfund Research Program

Kinetic, Ecological and Genetic Factors Affecting Bioaugmentation of Carbon Tetrachloride-Contaminated Sites with Pseudomonas sp. Strain KC

Project Leader: Craig S. Criddle (Stanford University)
Grant Number: P42ES004911
Funding Period: 1995 - 2000

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Final Progress Reports

Year:   1999 

Over the last year, work was completed in the carbon tetrachloride plume at Schoolcraft, MI. This project is one of the first field-scale demonstrations of bioaugmentation. The site was bioaugmented with Pseudomonas stutzeri KC and operated for over two years, exhibiting highly efficient removal of carbon tetrachloride. A paper in Ground Water (in press) describes the site and the design process. This approach should be broadly applicable at many sites of groundwater contamination. Additional papers will describe process performance, modeling, and changes in microbial ecology. Microcosm studies also provided important information over the past year. Researchers described the role of motility in the transformation of carbon tetrachloride and how a stationary biocurtain is established by strain KC. Currently, this data is being modeled, and the researchers are demonstrating that pyridine-2,6-(bis)thiocarboxylate (PDTC) is secreted by strain KC in microcosms that mimic field conditions.

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