Superfund Research Program
Cardiac Teratogenicity of Halogenated Hydrocarbons
Project Leader: Paula D. Johnson
Grant Number: P42ES004940
Funding Period: 1995 - 2000
During the past year, researchers characterized the expression of two molecules associated with exposure to trichloroethylene. The p137 and SERCA-like Ca++ATPase transcripts were found to be differentially expressed in rat embryonic heart (day 11) exposed to either TCE, or a metabolite (DCE and TCAA) through maternal drinking water, as compared with unexposed animals. The expression levels were analyzed by dot blot hybridization using dig-labeled probes for p137 or SERCA-like cDNA against aliquots of cDNA obtained from embryonic heart exposed to various concentrations of TCE (from 0.1 to 100 ppm). These experiments indicated that the expression level of both transcripts was down-regulated by increasing concentrations of TCE in a dose-response fashion. Further characterization of both molecules was then undertaken to prove their usefulness as markers of exposure to TCE and will provide new insight into the molecular mechanisms of TCE-induced birth defects.