Superfund Research Program

Molecular Biomarkers for Assessing Testicular Toxicity

Project Leader: Kim Boekelheide
Grant Number: P42ES013660
Funding Period: 2009-2021

Project-Specific Links

Publications

2021

• Bianchi E, Stermer A, Nolan T, Li H, Hall SJ, Boekelheide K, Sigman M, Hwang K. 2021. Highly conserved sperm function-related transcripts across three species: Human, rat and mouse. Reprod Toxicol 104:44-51. doi:10.1016/j.reprotox.2021.06.012 PMID:34174366

2019

• Bianchi E, Boekelheide K, Sigman M, Braun JM, Eliot M, Hall SJ, Dere E, Hwang K. 2019. Spermatozoal large RNA content is associated with semen characteristics, sociodemographic and lifestyle factors. PLoS One 14(5):doi:10.1371/journal.pone.0216584 PMID:31120914 PMCID:PMC6532849
• Dent MP, Madnick SJ, Hall SJ, Vantangoli Policelli M, Bars C, Li H, Amin A, Carmichael PL, Martin FL, Boekelheide K. 2019. A human-derived prostate co-culture microtissue model using epithelial (RWPE-1) and stromal (WPMY-1) cell lines. Toxicol In Vitro 60:203-211. doi:10.1016/j.tiv.2019.05.023 PMID:31154061 PMCID:PMC6717042
• Spade DJ, Dere E, Hall SJ, Schorl C, Freiman RN, Boekelheide K. 2019. All-Trans Retinoic Acid Disrupts Development in Ex Vivo Cultured Fetal Rat Testes. I: Altered Seminiferous Cord Maturation and Testicular Cell Fate. Toxicol Sci 167:546-558. doi:10.1093/toxsci/kfy260 PMID:30329139 PMCID:PMC6358251
• Stermer A, Klein D, Wilson S, Dalaijamts C, Bai CY, Hall SJ, Madnick SJ, Bianchi E, Chiu WA, Boekelheide K. 2019. Differential toxicity of water versus gavage exposure to trichloroethylene in rats. Environ Toxicol Pharmacol 68:1-3. doi:10.1016/j.etap.2019.02.003 PMID:30836291 PMCID:PMC6594756
• Stermer A, Reyes G, Hall SJ, Boekelheide K. 2019. Small RNAs in rat sperm are a predictive and sensitive biomarker of exposure to the testicular toxicant ethylene glycol monomethyl ether. Toxicol Sci 169(2):399-408. doi:10.1093/toxsci/kfz041 PMID:30768127 PMCID:PMC6934889
• Stermer A, Wilson SK, Klein D, Hall SJ, Boekelheide K. 2019. Trichloroethylene exposure alters dimethylated histone three lysine four in protein kinase A signaling pathway chromatin of rat sperm. Biol Reprod 101(5):875-877. doi:10.1093/biolre/ioz155 PMID:31426087 PMCID:PMC730579

2018

• Bianchi E, Stermer A, Boekelheide K, Sigman M, Hall SJ, Reyes G, Dere E, Hwang K. 2018. High-quality human and rat spermatozoal RNA isolation for functional genomic studies. J Androl 6:374-383. doi:10.1111/andr.12471 PMID:29470852 PMCID:PMC6309170
• Spade DJ, Bai CY, Lambright C, Conley JM, Boekelheide K, Gray LE. 2018. Validation of an automated counting procedure for phthalate-induced testicular multinucleated germ cells. Toxicol Lett 290:55-61. doi:10.1016/j.toxlet.2018.03.018 PMID:29571896 PMCID:PMC5921076
• Wilson S, Dere E, Klein D, Schorl C, Hall SJ, Boekelheide K. 2018. Localization of dimethylated histone three lysine four in the Rattus norvegicus sperm genome. Biol Reprod 99(2):266-268. doi:10.1093/biolre/ioy051 PMID:29506216

2016

• Dere E, Huse SM, Hwang K, Sigman M, Boekelheide K. 2016. Intra- and inter-individual differences in human sperm DNA methylation. Andrology 4(5):832-842. doi:10.1111/andr.12170 PMID:27089098 PMCID:PMC4988918
• Dere E, Wilson SK, Anderson LM, Boekelheide K. 2016. From the cover: sperm molecular biomarkers are sensitive indicators of testicular injury following subchronic model toxicant exposure. Toxicol Sci 153(2):327-340. doi:10.1093/toxsci/kfw137 PMID:27466211 PMCID:PMC5036622

2015

• Hofmann JW, Zhao X, De Cecco M, Peterson AL, Pagliaroli L, Manivannan J, Hubbard GB, Ikeno Y, Zhang Y, Feng B, Li X, Serre T, Qi W, Van Remmen H, Miller RA, Bath KG, de Cabo R, Xu H, Neretti N, Sedivy JM. 2015. Reduced expression of MYC increases longevity and enhances healthspan. Cell 160(3):477-488. doi:10.1016/j.cell.2014.12.016 PMID:25619689 PMCID:PMC4624921

2014

• Catlin NR, Huse SM, Boekelheide K. 2014. The stage-specific testicular germ cell apoptotic response to low-dose X-irradiation and 2,5-hexanedione combined exposure I: Validation of the laser capture microdissection method for qRT-PCR array application. Toxicol Pathol 42(8):1221-1228. doi:10.1177/0192623314526319 PMID:24717900 PMCID:PMC4192118
• Catlin NR, Huse SM, Boekelheide K. 2014. The stage-specific testicular germ cell apoptotic response to low-dose radiation and 2,5-hexanedione combined exposure II: qRT-PCR array analysis reveals dose dependent adaptive alterations in the apoptotic pathway. Toxicol Pathol 42(8):1229-1237. doi:10.1177/0192623314525689 PMID:24670816 PMCID:PMC4177022
• Rider CV, Boekelheide K, Catlin NR, Gordon CJ, Morata T, Selgrade MK, Sexton K, Simmons JE. 2014. Cumulative risk: toxicity and interactions of physical and chemical stressors. Toxicol Sci 137(1):3-11. doi:10.1093/toxsci/kft228 PMID:24154487 PMCID:PMC3871930

2013

• Dere E, Anderson LM, Coulson M, McIntyre BS, Boekelheide K, Chapin RE. 2013. SOT symposium highlight: translatable indicators of testicular toxicity: inhibin B, microRNAs, and sperm signatures. Toxicol Sci 136(2):265-273. doi:10.1093/toxsci/kft207 PMID:24052563 PMCID:PMC3858194
• Dere E, Anderson LM, Hwang K, Boekelheide K. 2013. Biomarkers of chemotherapy-induced testicular damage. Fertil Steril 100(5):1192-1202. doi:10.1016/j.fertnstert.2013.09.017 PMID:24182554 PMCID:PMC3840355
• Thompson MR, Boekelheide K. 2013. Multiple environmental chemical exposures to lead, mercury and polychlorinated biphenyls among childbearing-aged women (NHANES 1999–2004): Body burden and risk factors. Environ Res 121:23-30. doi:10.1016/j.envres.2012.10.005 PMID:23158727 PMCID:PMC3578119

2012

• Campion SN, Catlin NR, Houseman E, Hensley J, Sui Y, Gaido KW, Wu Z, Boekelheide K. 2012. Molecular alterations underlying the enhanced disruption of spermatogenesis by 2,5-hexanedione and carbendazim co-exposure. Reprod Toxicol 33:382-389. doi:10.1016/j.reprotox.2012.01.014 PMID:22382377 PMCID:PMC3327768
• Pacheco SE, Anderson LM, Boekelheide K. 2012. Optimization of a filter-lysis protocol to purify rat testicular homogenates for automated spermatid counting. J Androl 33(5):811-816. doi:10.2164/jandrol.111.015131 PMID:22240558 PMCID:PMC3598013
• Pacheco SE, Anderson LM, Sandrof MA, Vantangoli MM, Hall SJ, Boekelheide K. 2012. Sperm mRNA transcripts are indicators of sub-chronic low dose testicular injury in the Fischer 344 rat. PLoS One 7:e44280. doi:10.1371/journal.pone.0044280 PMID:22952946 PMCID:PMC3432073
• Springer C, Dere E, Hall SJ, McDonnell EV, Roberts SC, Butt CM, Stapleton HM, Watkins DJ, McClean MD, Webster TF, Schlezinger JJ, Boekelheide K. 2012. Rodent thyroid, liver, and fatal testis toxicity of the monoester metabolite of Bis-(2-ethylhexyl) Tetrabromophthalate (TBPH), a novel brominated flame retardant present in indoor dust. Environ Health Perspect 120:1711-1719. doi:10.1289/ehp.1204932 PMID:23014847 PMCID:PMC3548273

2011

• Marsit CJ, Koestler DC, Christensen BC, Karagas MR, Houseman EA, Kelsey KT. 2011. DNA methylation array analysis identifies profiles of blood-derived DNA methylation associated with bladder cancer. J Clin Oncol 29(9):1133-1139. doi:10.1200/JCO.2010.31.3577 PMID:21343564 PMCID:PMC3083868 (Completed with ARRA Funds)

2010

• Boekelheide K, Campion SN. 2010. Toxicity testing in the 21st century: using the new toxicity testing paradigm to create a taxonomy of adverse effects. Toxicol Sci 114(1):20-24. doi:10.1093/toxsci/kfp307 PMID:20026472 PMCID:PMC2902917
• Campion SN, Sandrof MA, Hensley J, Sui Y, Gaido KW, Boekelheide K. 2010. Suppression of radiation-induced testicular germ cell apoptosis by 2,5-hexanedione pretreatment. II. Gene array analysis reveals adaptive changes in cell cycle and cell death pathways. Toxicol Sci 117(2):457-465. doi:10.1093/toxsci/kfq204 PMID:20616210 PMCID:PMC2940405
• Campion SN, Sandrof MA, Yamasaki H, Boekelheide K. 2010. Suppression of radiation-induced testicular germ cell apoptosis by 2,5-hexanedione pretreatment. III. Candidate gene analysis identifies a role for fas in the attenuation of X-ray-induced apoptosis. Toxicol Sci 117(2):466-474. doi:10.1093/toxsci/kfq205 PMID:20616204 PMCID:PMC2940406
• Yamasaki H, Sandrof MA, Boekelheide K. 2010. Suppression of radiation-induced testicular germ cell apoptosis by 2,5-hexanedione pretreatment. I. Histopathological analysis reveals stage dependence of attenuated apoptosis. Toxicol Sci 117(2):449-456. doi:10.1093/toxsci/kfq203 PMID:20616207 PMCID:PMC2940404