Superfund Research Program
Impact of Airborne Heavy Metals on Lung Disease and the Environment
Center Director: Veena Antony
Grant Number: P42ES027723
Funding Period: 2020-2025
Program Links
Title: Discovery proteomics by mass spectrometry comparing secreted proteins from young and old mouse lung mesenchymal stromal cells
Accession Number: doi: 10.5061/dryad.x0k6djhj1
Link to Dataset: https://doi.org/10.5061/dryad.x0k6djhj1
Repository: Dryad
Data Type(s): Proteomics
Summary: Lung mesenchymal stromal cells (L-MSCs) were isolated from the young ( 3 months) and old (22-24 months) mice (N = 4 each) following collagenase digestion of the lungs and anchorage-dependent growth. Culture media was collected from young and old L-MSCs (10^6 cells) grown in culture for 24 hrs in serum-free condition. Culture media was then centrifuged to remove any cellular debris. Five (5) ml of supernatants were concentrated using 3kDa Molecular weight cut-off filters, and 10 micrograms of proteins were run as multiple MW 1D PAGE fractions using a standard GeLC approach with a nano-HPLC in-line with a Velos Pro Orbitrap MS. Individual data files were searched using Sequest, the results were then combined for each sample, followed by grouping, filtering, and quantifying by normalized spectral counts.
Publication(s) associated with this dataset:- Chanda D, Rehan M, Smith S, Dsouza K, Bernard K, Memula V, Kojima K, Mobley JA, Benavides G, Darley-Usmar VM, Kim Y, Zmijewski J, Deshane J, De Langhe S, Thannickal V. 2021. Mesenchymal stromal cell aging impairs the self-organizing capacity of lung alveolar epithelial stem cells. Elife doi:10.7554/eLife.68049 PMID:34528872 PMCID:PMC8445616