Superfund Research Program

Evan P. Gallagher

University of Washington
Department of Environmental and Occupational Health Sciences
Box 354695
Seattle, Washington 98105
Phone: 206-616-4739
Fax: 206-685-4696
Email: evang3@u.washington.edu

Projects

• University of Florida: Aquatic Toxicology Core (2000-2006)
• University of Washington: Administrative Core (1995-2023)
• University of Washington: Arsenic in Shallow Unstratified and Seasonally Stratified Urban Lakes: Mobility, Bioaccumulation and Ecological Toxicity (2015-2023)
• University of Washington: Cellular and Molecular Mechanisms of Cadmium Neurotoxicity (2015-2023)
• University of Washington: Gene-Environment Interactions in Salmon Neurotoxicity (2006-2009)
• University of Washington: Mechanisms and Biomarkers of Metal Olfactory Injury in Salmon (2009-2023)
• University of Washington: Training Core (2015-2023)

Research Briefs

• 269 - Platform Allows Rapid Analysis of Antioxidant Genes in Zebrafish
• 224 - Effects of Cadmium and Copper on the Fish Olfactory System

Hazardous Waste Sites

Commencement Bay, Near Shore/Tide Flats - Hylebos Waterway

• University of Washington: Gene-Environment Interactions in Salmon Neurotoxicity

Lower Duwamish Waterway

• University of Washington: Mechanisms and Biomarkers of Metal Olfactory Injury in Salmon

Publications

2020

• Putman NF, Williams CR, Gallagher EP, Dittman AH. 2020. A sense of place: Pink salmon use a magnetic map for orientation. J Exp Bio doi:10.1242/jeb.218735
• Steele WB, Kristofco LA, Corrales J, Saari GN, Corcoran EJ, Hill BN, Mills MG, Gallagher EP, Kavanagh TJ, Melnikov F, Zimmerman JB, Voutchkova-Kostal A, Anastas P, Kostal J, Brooks BW. 2020. Toward less hazardous industrial compounds: Coupling quantum mechanical computations, biomarker responses, and behavioral profiles to identify bioactivity of S(N)2 electrophiles in alternative vertebrate models. Chem Res Toxicol 33(2):367-380. doi:10.1021/acs.chemrestox.9b00290 PMID:31789507

2019

• Melnikov F, Botta D, White CC, Schmuck S, Winfough M, Schaupp C, Gallagher EP, Brooks BW, Williams E, Coish P, Anastas P, Voutchkova-Kostal A, Kostal J, Kavanagh TJ. 2019. Kinetics of Glutathione Depletion and Antioxidant Gene Expression as Indicators of Chemical Modes of Action Assessed in vitro in Mouse Hepatocytes with Enhanced Glutathione Synthesis. Chem Res Toxicol 32(3):421-436. doi:10.1021/acs.chemrestox.8b00259
• Mills MG, Ramsden R, Ma EY, Corrales J, Kristofco LA, Steele WB, Saari GN, Melnikov F, Kostal J, Kavanagh TJ, Zimmerman JB, Voutchkova-Kostal A, Brooks BW, Coish P, Anastas P, Gallagher EP. 2019. CRISPR-generated Nrf2a loss- and gain-of-function mutants facilitate mechanistic analysis of chemical oxidative stress-mediated toxicity in zebrafish. Chem Res Toxicol doi:10.1021/acs.chemrestox.9b00346

2018

• Heffern K, Tierney KB, Gallagher EP. 2018. Comparative effects of cadmium, zinc, arsenic and chromium on olfactory-mediated neurobehavior and gene expression in larval zebrafish (Danio rerio). Aquat Toxicol 201:83-90. doi:10.1016/j.aquatox.2018.05.016 PMID:29890505 PMCID:PMC6062444
• Ma EY, Heffern K, Cheresh J, Gallagher EP. 2018. Differential copper-induced death and regeneration of olfactory sensory neuron populations and neurobehavioral function in larval zebrafish. Neurotoxicology 69:141-151. doi:10.1016/j.neuro.2018.10.002 PMID:30292653
• Meador J, Yeh A, Gallagher EP. 2018. Adverse metabolic effects in fish exposed to contaminants of emerging concern in the field and laboratory. Environ Pollut 236:850-861. doi:10.1016/j.envpol.2018.02.007 PMID:29471284 PMCID:PMC5858870
• Steele WB, Kristofco LA, Corrales J, Saari GN, Haddad SP, Gallagher EP, Kavanagh TJ, Kostal J, Zimmerman JB, Voutchkova-Kostal A, Anastas P, Brooks BW. 2018. Comparative behavioral toxicology with two common larval fish models: exploring relationships among modes of action and locomotor responses. Sci Total Environ 640-641:1587-1600. doi:10.1016/j.scitotenv.2018.05.402 PMID:30021323
• Williams CR, Dittman AH, McElhany P, Busch DS, Maher MT, Bammler TK, MacDonald JW, Gallagher EP. 2018. Elevated CO2 impairs olfactory mediated neural and behavioral responses and gene expression in ocean-phase coho salmon (Oncorhynchus kisutch). Glob Chang Biol 25(3):963-977. doi:10.1111/gcb.14532 PMID:30561876 PMCID:PMC7065673

2017

• Meador J, Yeh A, Gallagher EP. 2017. Determining potential adverse effects in marine fish exposed to pharmaceuticals and personal care products with the fish plasma model and whole-body tissue concentrations. Environ Pollut 230:1018-1029. doi:10.1016/j.envpol.2017.07.047 PMID:28764109 PMCID:PMC5595653
• Mills MG, Gallagher EP. 2017. A targeted gene expression platform allows for rapid analysis of chemical-induced antioxidant mRNA expression in zebrafish larvae. PLoS One 12(2):e0171025. doi:10.1371/journal.pone.0171025 PMID:28212397 PMCID:PMC5315391
• Yeh A, Marcinek DJ, Meador J, Gallagher EP. 2017. Effect of contaminants of emerging concern on liver mitochondrial function in Chinook salmon. Aquat Toxicol 190:21-31. doi:10.1016/j.aquatox.2017.06.011 PMID:28668760 PMCID:PMC5590637

2016

• Meador J, Yeh A, Young G, Gallagher EP. 2016. Contaminants of emerging concern in a large temperate estuary. Environ Pollut 213:254-267. doi:10.1016/j.envpol.2016.01.088 PMID:26907702
• Ramsden R, Gallagher EP. 2016. Dual NRF2 paralogs in Coho salmon and their antioxidant response element targets. Redox Biol 9:114-123. doi:10.1016/j.redox.2016.07.001 PMID:27470083 PMCID:PMC5068245
• Wang L, Espinoza HM, MacDonald JW, Bammler TK, Williams CR, Yeh A, Louie KW, Marcinek DJ, Gallagher EP. 2016. Olfactory transcriptional analysis of salmon exposed to mixtures of chlorpyrifos and malathion reveal novel molecular pathways of neurobehavioral injury. Toxicol Sci 149(1):145-157. doi:10.1093/toxsci/kfv223 PMID:26494550 PMCID:PMC4731405
• Williams CR, MacDonald JW, Bammler TK, Simpson C, Paulsen M, Gallagher EP. 2016. From the cover: cadmium exposure differentially alters odorant-driven behaviors and expression of olfactory receptors in juvenile Coho salmon (Oncorhynchus kisutch). Toxicol Sci 154(2):267-277. doi:10.1093/toxsci/kfw172 PMID:27621283 PMCID:PMC5139070

2015

• Maryoung LA, Lavado R, Bammler TK, Gallagher EP, Stapleton PL, Beyer RP, Farin FM, Hardiman G, Schlenk D. 2015. Differential gene expression in liver, gill, and olfactory rosettes of Coho salmon (Oncorhynchus kisutch) after acclimation to salinity. Mar Biotechnol (NY) 17(6):703-717. doi:10.1007/s10126-015-9649-5 PMID:26260986 PMCID:PMC4636457

2014

• Hook SE, Gallagher EP, Batley GE. 2014. The role of biomarkers in the assessment of aquatic ecosystem health. Integr Environ Assess Manag 10(3):327-341. doi:10.1002/ieam.1530 PMID:24574147 PMCID:PMC4750648

2013

• Espinoza HM, Shireman LM, McClain V, Atkins W, Gallagher EP. 2013. Cloning, expression and analysis of the olfactory glutathione S-transferases in coho salmon. Biochem Pharmacol 85(6):839-848. doi:10.1016/j.bcp.2012.11.018 PMID:23261526 PMCID:PMC3660137
• Wang L, Bammler TK, Beyer RP, Gallagher EP. 2013. Copper-induced deregulation of microRNA expression in the zebrafish olfactory system. Environ Sci Technol 47(13):7466-7474. doi:10.1021/es400615q PMID:23745839 PMCID:PMC4750873
• Wang L, Espinoza HM, Gallagher EP. 2013. Brief exposure to copper induces apoptosis and alters mediators of olfactory signal transduction in coho salmon. Chemosphere 93(10):2639-2643. doi:10.1016/j.chemosphere.2013.08.044 PMID:24050714 PMCID:PMC3840796
• Wang L, Gallagher EP. 2013. Role of Nrf2 antioxidant defense in mitigating cadmium-induced oxidative stress in the olfactory system of zebrafish. Toxicol Appl Pharmacol 266(2):177-186. doi:10.1016/j.taap.2012.11.010 PMID:23174481
• Williams CR, Gallagher EP. 2013. Effects of cadmium on olfactory mediated behaviors and molecular biomarkers in coho salmon (Oncorhynchus kisutch). Aquat Toxicol 140-141:295-302. doi:10.1016/j.aquatox.2013.06.010 PMID:23851056 PMCID:PMC3770528

2012

• Harris SM, Espinoza HM, McClain V, Gallagher EP, Wang L. 2012. Characterization of phospholipid hydroperoxide glutathione metabolizing peroxidase (gpx4) isoforms in Coho Salmon olfactory and liver tissues and their modulation by cadmium. Aquat Toxicol 114-115:134-141. doi:10.1016/j.aquatox.2012.02.025 PMID:22446825 PMCID:PMC3660139
• McClain V, Stapleton HM, Tilton F, Gallagher EP. 2012. BDE 49 and developmental toxicity in zebrafish. Comp Biochem Physiol C Toxicol Pharmacol 155:253-258. doi:10.1016/j.cbpc.2011.09.004 PMID:21951712 PMCID:PMC3246048

2011

• Tilton F, Bammler TK, Gallagher EP. 2011. Swimming impairment and acetylcholinesterase inhibition in zebrafish exposed to copper or chlorpyrifos separately, or as mixtures. Comp Biochem Physiol C Toxicol Pharmacol 153(1):9-16. doi:10.1016/j.cbpc.2010.07.008 PMID:20692364 PMCID:PMC3034093
• Tilton F, Tilton SC, Bammler TK, Beyer RP, Stapleton PL, Scholz N, Gallagher EP. 2011. Transcriptional impact of organophosphate and metal mixtures on olfaction: Copper dominates the chlorpyrifos-induced response in adult zebrafish. Aquat Toxicol 102(3-4):205-215. doi:10.1016/j.aquatox.2011.01.012 PMID:21356183 PMCID:PMC3991301

2010

• Browne E, Kelley MA, Zhou GD, He L, McDonald TJ, Wang S, Duncan B, Meador J, Donnelly KC, Gallagher EP. 2010. In situ biomonitoring of juvenile Chinook salmon (Onchorhynchus tshawytscha) using biomarkers of chemical exposures and effects in a partially remediated urbanized waterway of the Puget Sound, WA. Environ Res 110(7):675-683. doi:10.1016/j.envres.2010.06.007 PMID:20619832 PMCID:PMC3321373
• Shao J, Dabrowski MJ, White CC, Kavanagh TJ, Gallagher EP. 2010. Flow cytometric analysis of BDE 47 mediated injury to rainbow trout gill epithelial cells. Aquat Toxicol 97(1):42-50. doi:10.1016/j.aquatox.2009.11.013 PMID:20053465 PMCID:PMC3321377

2009

• Lefebvre KA, Tilton SC, Bammler TK, Beyer RP, Srinouanprachanh SL, Stapleton PL, Farin FM, Gallagher EP. 2009. Gene expression profiles in zebrafish brain after acute exposure to domoic acid at symptomatic and asymptomatic doses. Toxicol Sci 107(1):65-77. doi:10.1093/toxsci/kfn207 PMID:18936300 PMCID:PMC2735419

2008

• Gallagher EP, Stapleton PL, Bammler TK, Beyer RP, Farin FM, LaVire HM. 2008. Hepatic expression profiling in smolting and adult coho salmon (Onchorhynchus kisutch). Environ Res 106(3):365-378. doi:10.1016/j.envres.2007.10.001 PMID:18241857 PMCID:PMC3321376
• Matsuo AY, Gallagher EP, Trute M, Schlenk D, Stapleton PL, Levado R. 2008. Characterization of Phase I biotransformation enzymes in coho salmon (Oncorhynchus kisutch). Comp Biochem Physiol C Toxicol Pharmacol 147(1):78-84. doi:10.1016/j.cbpc.2007.08.001 PMID:17826357 PMCID:PMC2694037
• Tilton F, Tilton SC, Bammler TK, Beyer RP, Farin FM, Stapleton PL, Gallagher EP. 2008. Transcriptional biomarkers and mechanisms of copper-induced olfactory injury in zebrafish. Environ Sci Technol 42(24):9404-11. doi:10.1021/es801636v PMID:19174923

2007

• Carvan MJ, Gallagher EP, Goksoyr A, Hahn ME, Joakim DG. 2007. Fish models in toxicology. Zebrafish 4(1):9-20. doi:10.1089/zeb.2006.9998 PMID:18041939
• Gallagher EP, Huisden CM, Gardner JL. 2007. Transfection of HepG2 cells with hGSTA4 provides protection against 4-hydroxynonenal-mediated oxidative injury. Toxicol In Vitro 21(8):1365-72. PMID:17553661
• Trute M, Gallis B, Doneanu C, Shaffer S, Goodlett DR, Gallagher EP. 2007. Characterization of hepatic glutathione S-transferases in coho salmon (Oncorhynchus kisutch). Aquat Toxicol 81(2):126-136. PMID:17184855
• Trute M, Gallus B, Goodlett DR, Gallagher EP. 2007. Glutathione S-transferase isozyme expression in coho salmon liver. Aquat Toxicol 81:126-136.

2006

• Richardson K, Gardner S, Gallagher EP, Schlenk D. 2006. Halogenated contaminants and detoxification enzymes in turtles from the Pacific Ocean. In: 27^th Annual Meeting of the Society of Environmental Toxicology and Chemistry (SETAC) North America; November 5-9. Montreal, Canada.

2004

• Doi AM, Pham RT, Hughes EM, Barber DS, Gallagher EP. 2004. Molecular cloning and characterization of a glutathione S-transferase from largemouth bass (Micropterus salmoides) liver that is involved in the detoxification of 4-hydroxynonenal. Biochem Pharmacol 67(11):2129-2139. doi:10.1016/j.bcp.2004.01.024 PMID:15135309
• Henson KL, Gallagher EP. 2004. Glutathione S-transferase expression in pollution-associated hepatic lesions of brown bullheads (Ameiurus nebulosus) from the Cuyahoga River, Cleveland, Ohio. Toxicol Sci 80(1):26-33. PMID:15071171
• Hughes EM, Gallagher EP. 2004. Effects of 4-nonylphenol and 17 Beta-estradiol on phase two antioxidant gene expression in largemouth bass liver. Comp Biochem Physiol C Toxicol Pharmacol 137(3):237-247.
• Hughes EM, Gallagher EP. 2004. Effects of beta-naphthoflavone on hepatic biotransformation and glutathione biosynthesis in largemouth bass (Micropterus salmoides). Mar Environ Res 58(2-5):675-679. doi:10.1016/j.marenvres.2004.03.061 PMID:15178098
• Pham RT, Barber DS, Gallagher EP. 2004. GSTA is the major gene responsible for 4-hydroxynonenal metabolism in largemouth bass liver. Mar Environ Res 58(2-5):485-488. PMID:15178070
• Sepulveda MS, Gallagher EP, Gross TS. 2004. Effects of pulp mill effluents on health and reproductive parameters and in largemouth bass. Environ Sci Technol 13:291-301.
• Sepulveda MS, Gallagher EP, Wieser CM, Gross TS. 2004. Reproductive and biochemical biomarkers in largemouth bass sampled downstream of a pulp and paper mill in Florida. Ecotoxicol Environ Saf 57(3):431-440. doi:10.1016/j.ecoenv.2003.08.020 PMID:15041266

2002

• Doi AM, Gallagher EP. 2002. Isolation and cloning of a major glutathione S-transferase from largemouth bass (Micropterus salmoides). In: Proceedings of the 41^st Annual Meeting of the Society of Toxicology (SOT), March 17-21, 2002. Nashville, TN.
• Gallagher EP. 2002. Cloning and expression of a novel glutathione S-transferase that provides protection against oxidative injury in largemouth bass liver. In: Proceedings of the Aquatic Toxicology Workshop. Whistler, British Columbia.
• Gallagher EP. 2002. Conservation of a glutathione S-transferase that provides protection against oxidative injury in largemouth bass. In: Proceedings of the Society of Environmental Toxicology and Chemistry (SETC), 23^rd Annual Meeting, Salt Lake City, Utah. November 16 - 20.
• Hughes EM, Gallagher EP. 2002. Effects of natural and synthetic extrogens on largemouth bass hepatic oxidant defense pathways. In: Proceedings of the Society of Environmental Toxicology and Chemistry (SETC), 23^rd Annual Meeting, Salt Lake City, Utah. November 16 - 20.
• Pham RT, Gardner JL, Gallagher EP. 2002. Conjugation of 4-hydroxynonenal by largemouth bass (Micropterus salmoides) glutathione S-transferases. Mar Environ Res 54(3-5):291-295. PMID:12408579

2001

• Gallagher EP, Gross TS, Sheehy KM. 2001. Decreased glutathione S-transferase expression and activity and altered sex steroids in Lake Apopka brown bullheads (Ameriurus nebulosus). Aquat Toxicol 55(3-4):223-237. PMID:11595311
• Henson KL, Stauffer G, Gallagher EP. 2001. Induction of glutathione S-transferase activity and protein expression in brown bullhead (Ameriurus nebulosus) liver by ethoxyquin. Toxicol Sci 62(1):54-60. PMID:11399793

1998

• Sepulveda MS, Gallagher EP, Schoeb TR, Denslow ND, Holm SE, Gross DA, Ruessler S, Gross TS. 1998. Effects of Paper Mill Effluents on Reproduction and Health of Largemouth Bass (Micropterus salmoides) in Florida. In: Proceedings of the Society of Environmental Toxicology and Chemistry (SETC) 19^th Annual Meeting. Charlotte, NC.

1997

• Reid LL, Botta D, Lu Y, Gallagher EP, Kavanagh TJ. 1997. Molecular cloning and sequencing of the cDNA encoding the catalytic subunit of mouse glutamate-cysteine ligase. Biochim Biophys Acta 1352(3):233-237. PMID:9224945

1996

• Gallagher EP, Stapleton PL, Slone DH, Schlenk D, Eaton DL. 1996. Channel catfish glutathione S-transferase isoenzyme activity toward (+)-anti-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide. Aquat Toxicol 34(2):135-150.

1995

• Buetler TM, Gallagher EP, Wang C, Stahl DL, Hayes JD, Eaton DL. 1995. Induction of phase I and phase II drug metabolizing enzyme mRNA, protein and activity by BHA, ethoxyquin and oltipraz. Toxicol Appl Pharmacol 135(1):45-57. PMID:7482539
• Gallagher EP, Buetler TM, Wang C, Stapleton PL, Stahl DL, Eaton DL. 1995. The effects of diquat and ciprofibrate on mRNA expression and catalytic activities of hepatic xenobiotic metabolizing and antioxidant enzymes in rat liver. Toxicol Appl Pharmacol 134(1):81-91. PMID:7676460
• Gallagher EP, Eaton DL. 1995. In vitro biotransformation of aflatoxin B1 in channel catfish liver. Toxicol Appl Pharmacol 132:82-90. PMID:7747288