Superfund Research Program
Research Support Core B: Environmental Molecular Analysis Core
Project Leader: James M. Tiedje
Grant Number: P42ES004911
Funding Period: 2006-2020
The Environmental Molecular Analysis Core has worked with Molecular Insight into Dioxin Degradation by Microbes and Microbial Communities and Geochemical Controls on the Sorption, Bioavailability, Formation, and Long-term Environmental Fate of Polychlorinated Dibenzo-p-Dioxins (PCDDs) Projects to analyze changes in RW-1, a pure-culture dioxin degrader, when challenged by either free TCDD or biochar-bound to TCDD. The Core found that the biochar-bound TCDD is less toxic to RW-1. They are analyzing RW-1 transcriptomes collected under these conditions. The Core continues to support the Molecular Insight into Dioxin Degradation by Microbes and Microbial Communities Project in the development of a qPCR array targeting dechlorinases, a very diverse category of genes involved in removal of chlorines from TCDD and other chlorinated pollutants. This provides the researchers with a genetic test to track these important genes in the environment.