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Project Publications: Michigan State University: Geochemical Controls on the Adsorption, Bioavailability, Formation, and Long-term Environmental Fate of Polychlorinated Dibenzo-p-Dioxins (PCDDs)

Superfund Research Program

Geochemical Controls on the Adsorption, Bioavailability, Formation, and Long-term Environmental Fate of Polychlorinated Dibenzo-p-Dioxins (PCDDs)

Project Leader: Stephen A. Boyd
Grant Number: P42ES004911
Funding Period: 2006-2021

Project-Specific Links




  • Chai B, Tsoi TV, Sallach JB, Liu C, Landgraf J, Bezdek M, Zylstra GJ, Li H, Johnston CT, Teppen BJ, Cole JR, Boyd SA, Tiedje JM. 2020. Bioavailability of clay-adsorbed dioxin to Sphingomonas wittichii RW1 and its associated genome-wide shifts in gene expression. Sci Total Environ 712:135525. doi:10.1016/j.scitotenv.2019.135525 PMID:32050392


  • Gao F, Hirn M, Perlmutter M, Wolf G. 2019. Geometric wavelet scattering on graphs and manifolds. Wavelets and Sparsity Xviii doi:10.1117/12.2529615
  • Sallach JB, Crawford RB, Li H, Johnston CT, Teppen BJ, Kaminski NE, Boyd SA. 2019. Activated carbons of varying pore structure eliminate the bioavailability of 2,3,7,8-tetrachlorodibenzo-p-dioxin to a mammalian (mouse) model. Sci Total Environ 650:2231-2238. doi:10.1016/j.scitotenv.2018.09.270


  • Fu H, Wei C, Qu X, Li H, Zhu D. 2018. Strong binding of apolar hydrophobic organic contaminants by dissolved black carbon released from biochar: a mechanism of pseudomicelle partition and environmental implications. Environ Pollut 232:402-410. doi:10.1016/j.envpol.2017.09.053 PMID:28966024
  • Liu C, Chu W, Li H, Boyd SA, Teppen BJ, Mao J, Lehmann J, Zhang W. 2018. Quantification and characterization of dissolved organic carbon from biochars. Geoderma 335:161-169. doi:10.1016/j.geoderma.2018.08.019
  • Peng A, Gao J, Chen Z, Wang Y, Li H, Ma LQ, Gu C. 2018. Interactions of gaseous 2-chlorophenol with Fe3+-saturated montmorillonite and their toxicity to human lung cells. Environ Sci Technol 52(9):5208-5217. doi:10.1021/acs.est.7b06664 PMID:29613777
  • Zhang S, Liu Q, Cheng H, Gao F, Liu C, Teppen BJ. 2018. Mechanism responsible for intercalation of dimethyl sulfoxide in kaolinite: Molecular dynamics simulations. Appl Clay Sci 151:46-53. doi:10.1016/j.clay.2017.10.022 PMID:29545655 PMCID:PMC5846688
  • Zhang S, Liu Q, Gao F, Ma R, Wu Z, Teppen BJ. 2018. Interfacial structure and interaction of kaolinite intercalated with N-methylformamide: insight from molecular dynamics modeling. Appl Clay Sci 158:204-210. doi:10.1016/j.clay.2018.03.032 PMID:30364591 PMCID:PMC6197067
  • Zhang S, Liu Q, Gao F, Teppen BJ. 2018. Molecular dynamics simulation of basal spacing, energetics, and structure evolution of a kaolinite-formamide intercalation complex and their interfacial interaction. Journal of Physical Chemistry C 122:3341-3349. doi:10.1021/acs.jpcc.7b10234 PMID:29657662 PMCID:PMC5896007
  • Zhang Y, Boyd SA, Teppen BJ, Tiedje JM, Zhang W, Zhu D, Li H. 2018. Bioavailability of tetracycline to antibiotic resistant Escherichia coli in water-clay systems. Environ Pollut 243(PtB):1078-1086. doi:10.1016/j.envpol.2018.09.059 PMID:30253298


  • Boyd SA, Sallach JB, Zhang Y, Crawford RB, Li H, Johnston CT, Teppen BJ, Kaminski NE. 2017. Sequestration of 2,3,7,8-tetrachlorodibenzo-p-dioxin by activated carbon eliminates bioavailability and the suppression of immune function in mice. Environ Toxicol Chem 36(10):2671-2678. doi:10.1002/etc.3815 PMID:28370362
  • Clemente J, Beauchemin S, MacKinnon T, Martin J, Johnston CT, Joern B. 2017. Initial biochar properties related to the removal of As, Se, Pb, Cd, Cu, Ni, and Zn from an acidic suspension. Chemosphere 170:216-224. doi:10.1016/j.chemosphere.2016.11.154 PMID:28006756
  • Schnetzer F, Johnston CT, Premachandra GS, Giraudo N, Schuhmann R, Thissen P, Emmerich K. 2017. Impact of intrinsic structural properties on the hydration of 2:1 layer silicates. ACS Earth Space Chem 1(10):608-620. doi:10.1021/acsearthspacechem.7b00091 PMID:29658010 PMCID:PMC5896019
  • Snow DD, Cassada DA, Bartelt-Hunt SL, Li X, D'Alessio M, Levine R, Zhang Y, Sallach JB. 2017. Detection, occurrence and fate of emerging contaminants in agricultural environments. Water Environ Res 89(10):897-920. doi:10.2175/106143015X14338845155101 PMID:26420073
  • Stedtfeld RD, Sallach JB, Crawford RB, Stedtfeld TM, Williams MR, Waseem H, Johnston CT, Li H, Teppen BJ, Kaminski NE, Boyd SA, Tiedje JM, Hashsham SA. 2017. TCDD administered on activated carbon eliminates bioavailability and subsequent shifts to a key murine gut commensal. Appl Microbiol Biotechnol 101:7409-7415. doi:10.1007/s00253-017-8460-9 PMID:28812142
  • Zhang S, Liu Q, Cheng H, Gao F, Liu C, Teppen BJ. 2017. Thermodynamic mechanism and interfacial structure of kaolinite intercalation and surface modification by alkane surfactants with neutral and ionic head groups. Journal of Physical Chemistry C 121:8824-8831. doi:10.1021/acs.jpcc.6b12919 PMID:29657661 PMCID:PMC5896017
  • Zhang S, Liu Q, Gao F, Li X, Liu C, Li H, Boyd SA, Johnston CT, Teppen BJ. 2017. Mechanism associated with kaolinite intercalation with urea: combination of infrared spectroscopy and molecular dynamics simulation studies. Journal of Physical Chemistry C 121(1):402-409. doi:10.1021/acs.jpcc.6b10533 PMID:29657660 PMCID:PMC5896021


  • Chai B, Tsoi TV, Iwai S, Liu C, Fish JA, Gu C, Johnson TA, Zylstra GJ, Teppen BJ, Li H, Hashsham SA, Boyd SA, Cole JR, Tiedje JM. 2016. Sphingomonas wittichii strain RW1 genome-wide gene expression shifts in response to dioxins and clay. PLoS One 11(6):e0157008. doi:10.1371/journal.pone.0157008 PMID:27309357 PMCID:PMC4911050
  • Gibson C, Berry TD, Wang R, Spencer JA, Johnston CT, Jiang Y, Bird JA, Filley TR. 2016. Weathering of pyrogenic organic matter induces fungal oxidative enzyme response in single culture inoculation experiments. Organic Geochemistry 92:32-41. doi:10.1016/j.orggeochem.2015.12.003
  • Haoting T, Gao J, Li H, Boyd SA, Gu C. 2016. Complete defluorination of perfluorinated compounds by hydrated electrons generated from 3-indole-acetic-acid in organomodified montmorillonite. Sci Rep 6:32949. doi:10.1038/srep32949 PMID:27608658 PMCID:PMC5017190
  • Johnston CT. 2016. Biochar analysis by fourier transform infrared analysis. Chapter 12 in: Methods of Biochar Analysis. CSIRO, Collingwood, Australia.
  • Liu C, Chuang Y, Li H, Teppen BJ, Boyd SA, Gonzalez J, Johnston CT, Lehmann J, Zhang W. 2016. Sorption of lincomycin by manure-derived biochars from water. J Environ Qual 45(2):519-527. doi:10.2134/jeq2015.06.0320 PMID:27065399
  • Masek O, Johnston CT. 2016. Thermal analysis for biochar characterization. Chapter 14 in: Methods of Biochar Analysis. CSIRO, Collingwood, Australia.
  • Singh B, Fang Y, Johnston CT. 2016. A fourier-transform infrared study of biochar aging in soils. Soil Science Society of America Journal 80:613-622. doi:10.2136/sssaj2015.11.0414


  • Tian H, Guo Y, Pan B, Gu C, Li H, Boyd SA. 2015. Enhanced photoreduction of nitro-aromatic compounds by hydrated electrons derived from indole on natural montmorillonite. Environ Sci Technol 49(13):7784-7792. doi:10.1021/acs.est.5b01026 PMID:26029791


  • Johnston CT, Khan B, Barth EF, Chattopadhyay S, Boyd SA. 2012. Nature of the interlayer environment in an organoclay optimized for the sequestration of dibenzo-p-dioxin. Environ Sci Technol 46(17):9584-9591. doi:10.1021/es300699y PMID:22856528 PMCID:PMC4327870
  • Liu C, Hui L, Johnston CT, Boyd SA, Teppen BJ. 2012. Relating clay structural factors to dioxin adsorption by smectites: molecular dynamics simulations. Soil Science Society of America Journal 76:110-120. doi:10.2136/sssaj2010.0450
  • Yu K, Gu C, Boyd SA, Liu C, Teppen BJ, Li H, Sun C. 2012. Rapid and extensive debromination of decabromodiphenyl ether by smectite clay-templated subnanoscale zero-valent iron. Environ Sci Technol 46(16):8969-8975. doi:10.1021/es300516e PMID:22846063


  • Boyd SA, Johnston CT, Pinnavaia TJ, Kaminski NE, Teppen BJ, Crawford RB, Kovalova N, Kim SS, Shao H, Gu C, Kaplan BL, Khan B, Li H. 2011. Suppression of humoral immune responses by 2,3,7,8-tetrachlorodibenzo-p-dioxin intercalated in smectite clay. Environ Toxicol Chem 30(12):2748-2755. doi:10.1002/etc.701 PMID:21994089
  • Gu C, Liu C, Ding Y, Li H, Teppen BJ, Johnston CT, Boyd SA. 2011. Clay mediated route to natural formation of Polychlorodibenzo-p-dioxins. Environ Sci Technol 45(8):3445-3451. doi:10.1021/es104225d PMID:21434682
  • Gu C, Liu C, Johnston CT, Teppen BJ, Li H, Boyd SA. 2011. Pentachlorophenol radical cations generated on Fe(III)-montmorillonite initiate octachlorodibenzo-p-dioxin formation in clays: density functional theory and fourier transform infrared studies. Environ Sci Technol 45(4):1399-1406. doi:10.1021/es103324z PMID:21254769 PMCID:PMC3094738
  • Jia H, Gu C, Boyd SA, Teppen BJ, Johnston CT, Li H, Song C. 2011. Comparison of reactivity of nanoscaled zero-valent iron formed on clay surfaces. Soil Science Society of America Journal 75(2):357-364. doi:10.2136/sssaj2010.0080nps
  • Kaplan BL, Crawford RB, Kovalova N, Arencibia A, Kim S, Pinnavaia TJ, Boyd SA, Teppen BJ, Kaminski NE. 2011. TCDD adsorbed on silica as a model for TCDD contaminated soils: evidence for suppression of humoral immunity in mice. Toxicology 282(3):82-87. doi:10.1016/j.tox.2011.01.013 PMID:21272611 PMCID:PMC3089373


  • Boyd SA, Johnston CT, Laird DA, Teppen BJ, Li H. 2010. Comprehensive Study of Organic Contaminant Adsorption by Clays: Methodologies, Mechanisms and Environmental Implications. In: Volume 3, Biophysico-Chemical Processes of Anthropogenic Organic Compounds in Environmental Systems. Wiley, New York, NY.
  • Gu C, Jiang H, Li H, Teppen BJ, Boyd SA. 2010. Synthesis of highly reactive subnano-sized zero-valent iron using smectite clay templates. Environ Sci Technol 44(11):4258-4263. doi:10.1021/es903801r PMID:20446730 PMCID:PMC2892840
  • Johnston CT. 2010. Probing the nanoscale architecture of clay minerals. Clays and Clay Minerals 45:245-279. doi:10.1180/claymin.2010.045.3.245
  • Shao H, Pinnavaia TJ. 2010. Synthesis and properties of nanoparticle forms saponite clay, cancrinite zeolite and phase mixtures thereof. Microporous Mesoporous Mater 133:10-17. doi:10.1016/j.micromeso.2010.04.002 PMID:21709774 PMCID:PMC3121892
  • Thiex NW, Chames MC, Loch-Caruso R. 2010. Tissue-specific induction of COX-2 and prostaglandins in lipopolysaccharide-stimulated extraplacental human gestational membranes in a 2-chamber transwell culture system. Reprod Sci 17(12):1120-1129. doi:10.1177/1933719110378344 PMID:20861394 PMCID:PMC3096444
  • Xue S, Pinnavaia TJ. 2010. Methylene-functionalized saponite: a new type of organoclay with CH(2) groups substituting for bridging oxygen centers in the tetrahedral sheet. Appl Clay Sci 48(1-2):60-66. doi:10.1016/j.clay.2009.11.006 PMID:20508733 PMCID:PMC2874865


  • Liu C, Li H, Teppen BJ, Jonhston CT, Boyd SA. 2009. Mechanisms associated with the high adsorption of dibenzo-p-dioxin from water by smectite clays. Environ Sci Technol 43(8):2777-83. doi:10.1021/es802381z PMID:19475949
  • Park I, Pinnavaia TJ. 2009. Large-pore mesoporous silica with three-dimensional wormhole framework structures. Microporous Mesoporous Mater 118(1-3):239. doi:10.1016/j.micromeso.2008.08.027 PMID:20126285 PMCID:PMC2663960
  • Prouzet E, Boissière C, Kim SS, Pinnavaia TJ. 2009. Roughness of mesoporous silica surfaces deduced from adsorption measurements. Microporous Mesoporous Mater 119:9-17. doi:10.1016/j.micromeso.2008.09.031
  • Rana K, Boyd SA, Teppen BJ, Li H, Liu C, Johnston CT. 2009. Probing the microscopic hydrophobicity of smectite surfaces A vibrational spectroscopic study of dibenzo-p-dioxin sorption to smectite. Phys Chem Chem Phys 11(16):2976-2985. doi:10.1039/b822635k PMID:19421512


  • Cheng G, Li H, Teppen BJ, Boyd SA. 2008. Octachlorodibenzodioxin formation on Fe(III)-montmorillonite clay. Environ Sci Technol 42(13):4758-4763. doi:10.1021/es7029834 PMID:18678002
  • Tsuneta T, Loch-Caruso R, Quensen JF, Boyd SA, Hanna M, Grindatti C. 2008. Stimulatory effects of a microbially dechlorinated polychlorinated biphenyl (PCB) mixture on rat uterine contraction in vitro. Environ Res 107(2):185-193. doi:10.1016/j.envres.2008.01.011 PMID:18359014 PMCID:PMC2699757


  • Jeong HY, Kim H, Hayes KF. 2007. Reductive dechlorination pathways of tetrachloroethylene and trichloroethylene and subsequent transformation of their dechlorination products by mackinawite (FeS) in the presence of metals. Environ Sci Technol 41(22):7736-7743. doi:10.1021/es0708518 PMID:18075082
  • Jeong HY, Klaue B, Blum JD, Hayes KF. 2007. Sorption of mercuric ion by synthetic nanocrystalline mackinawite (FeS). Environ Sci Technol 41(22):7699-7705. PMID:18075077
  • Ras R, Johnston CT, Schoonheydt RA. 2007. Relation between s-polarized and p-polarized internal reflection spectra: application for the spectral resolution of perpendicular vibrational modes. J Phys Chem A 111(36):8787-8791. PMID:17665884
  • Ras R, Umemura Y, Johnston CT, Yamagishi A, Schoonheydt RA. 2007. Ultrathin hybrid films of clay minerals. Phys Chem Chem Phys 9(8):918. PMID:17301882
  • Szabo T, Szekeres M, Dekany I, Jackers C, De Feyter S, Johnston CT, Schoonheydt RA. 2007. Layer-by-layer construction of ultrathin hybrid films with proteins and clay minerals. Journal of Physical Chemistry C 111(34):12730-12740.
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