Superfund Research Program

Molecular Biology and Proteomics Core

Project Leader: Joshua W. Hamilton (Marine Biological Laboratory)
Co-Investigator: Jack Bodwell
Grant Number: P42ES007373
Funding Period: 2000-2008

Project-Specific Links

Final Progress Reports

Year:   2007  2004 

This core is a Molecular Biology & Proteomics Shared Resource that continues to provide state-of-the-art services and technical support for various molecular biology, biochemistry, genomics and proteomics needs, including DNA and RNA oligonucleotide synthesis and custom labeling syntheses; high throughput automated DNA sequencing and DNA-protein foot printing; peptide synthesis and purification; protein sequencing; imaging support including phosphor imaging, x-ray film processing and electronic gel imaging; DNA array making and array reading capabilities; DNA array analysis; quantitative real-time PCR; and protein sequencing, 2D protein analysis, and both LCQ- and MALDI-TOF mass spectrometry. Dartmouth recently hired a proteomics faculty specialist, Dr. Scott Gerber, who was previously at Harvard Medical School.  He joined this program as the Associate Director of this core, and director of its proteomics services.  The physical spaces for this core have undergone extensive renovation and updating over the past academic year, and additional instrumentation has been acquired as part of Dr. Gerber’s recruitment to Dartmouth.  The two sub-components of this core, i.e., Molecular Biology (housed in the Vail/Remsen buildings of the medical school) and Proteomics (housed in new space in the new Rubin Cancer Center building), are now state-of-the-art.  Dartmouth also recently recruited genomics expert, Dr. Craig Tomlinson, from Cincinnati, where he was involved in that institution’s SBRP program.  He is heading a state-of-the-art genomics core that also supports the Dartmouth SBRP.  Similarly, Dr. Hamilton and his laboratory recently recruited a bioinformatics expert, Dr. Jason Moore, who heads the newly renovated and state-of-the-art bioinformatics and biostatistics core at Dartmouth.  The goal of this program is to begin integrating services, i.e., molecular biology, genomics, proteomics, bioinformatics, biostatistics, and biomarkers validation, into an “Integrated Biology” program that supports the full spectrum of modern biomedical research in these areas.  Core A, working with these other cores, continues to support ongoing studies in the projects: Arsenic as an Endocrine Disruptor and Arsenic and ABC Transporters, Arsenic Epidemiology, Biomarkers and Exposure Assessment, Toxic Metal Interactions with Cellular Proteins, and Trophic Transfer of Toxic Metals in Aquatic Food Webs. The goal over the coming year will be to expand and more fully integrate these capabilities.