Superfund Research Program
Bioinformatics and Molecular Modeling Core
The work of the Bioinformatics and Molecular Modeling Core primarily focused on the (meta-)analysis of the killifish- and zebrafish-based PCB exposure gene expression profiles generated from the Mechanisms and Impact of PCB Resistant Fish and A Novel Mechanism of Ortho-PCB-induced Toxicity: Targeting Nuclear Receptors in Brain of Fish Projects, and on their integration with publicly available data of PCB exposures in mice and humans. This work was also the main impetus for the pursuit of an administrative supplement aimed at developing web-based interoperability capabilities to support interactive sharing and querying of the data and the analysis results. The Core also worked closely with the Environmental PPARγ Pathway Activators: Multifaceted Metabolic Disruptors Impacting Adipose and Bone Homeostasis Project team towards the completion of an important study aimed at assessing the effects and mechanisms of action of metabolism chemical disruptors (MDCs), also known as adipogens. In this study, they used functional assays and high-throughput transcriptomics to profile a large compendium of known and suspected MDCs and to build predictive models of their adverse health effects. They also developed tools for the interactive web-based sharing of the data and of the analysis results. The data and interface capabilities are being integrated in a data repository that they are developing as part of a second administrative supplement in collaboration with the OSU SRP. This repository will integrate the results of past studies on chemical carcinogens with the study on MDCs and with OSU’s zebrafish-based chemical screens, and will make available all the data and the analysis results in an interactive web-based portal.