Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Internet Explorer is no longer a supported browser.

This website may not display properly with Internet Explorer. For the best experience, please use a more recent browser such as the latest versions of Google Chrome, Microsoft Edge, and/or Mozilla Firefox. Thank you.

Your Environment. Your Health.

Progress Reports: University of California-Berkeley: Genomics and Analytical Chemistry

Superfund Research Program

Genomics and Analytical Chemistry

Project Leader: Daniel K. Nomura
Co-Investigator: Martyn T. Smith
Grant Number: P42ES004705
Funding Period: 2006-2017
View this project in the NIH Research Portfolio Online Reporting Tools (RePORT)

Learn More About the Grantee

Visit the grantee's eNewsletter page Visit the grantee's Twitter page Visit the grantee's Facebook page

Progress Reports

Year:   2016  2014  2013  2011  2010  2009  2008  2007  2006 

Over the past year, the Genomic and Analytical Chemistry Core has been developing and applying an innovative chemoproteomic strategy termed Isotopic Tandem Orthogonal Proteolysis-enabled Activity-based Protein Profiling (isoTOP-ABPP) to map proteome-wide targets of various environmental chemicals to better understand their toxicological mechanisms. IsoTOP-ABPP uses reactivity-based chemical probes to map proteome-wide reactive, functional, and ligandable hotspots directly in complex proteomes. When used in a competitive manner, small-molecules can be competed against binding of reactivity-based probes to ligandable hotspots to identify targets and off-targets of environmental chemicals. The Core has used isoTOP-ABPP to recently characterize the toxicological mechanisms of the widely used herbicides, acetochlor and glyphosate. They show that both chemicals, acetochlor in its parent form and glyphosate through its metabolism to glyoxylate, inhibit the catalytic cysteines of several thiolases involved in fatty acid oxidation in vivo in mouse liver. They show that inhibition of these targets leads to a diversion of fatty acids away from degradation and towards other lipid pathways, including triglycerides leading to hepatic steatosis. These studies have been accepted to American Chemical Society (ACS) Chemical Biology and Cell Chemical Biology, and will be published in the very near future.

to Top