Skip Navigation

University of California-San Diego: Dataset Details, ID=E-GEOD-15476

Maintenance notice: We are currently addressing issues with broken links due to recent major website changes. We apologize for any inconvenience and appreciate your patience. Please contact brittany.trottier@niehs.nih.gov for assistance.

Superfund Research Program

Detection and Models of Toxicant Exposure

Center Director: Robert H. Tukey
Grant Number: P42ES010337
Funding Period: 2000-2023
View this project in the NIH Research Portfolio Online Reporting Tools (RePORT)

Program Links

Connect with the Grant Recipients

Visit the grantee's eNewsletter page Visit the grantee's Instagram page Visit the grantee's Facebook page

Title: Comparisons between liver tissues and freshly isolated hepatocytes from Ikk F/F and Ikk Dhep (Ikk -deleted) mice

Accession Number: E-GEOD-15476

Repository: ArrayExpress

Data Type(s): Gene Expression

Experiment Type(s): Transcription profiling by array

Organism(s): Mus musculus

Summary: CD74, a Type II membrane glycoprotein and MHC class II chaperone (Ii), is normally expressed by cells associated with the immune system. CD74 also forms heterodimers with CD44 to generate receptors to macrophage migration inhibitory factor (MIF), a proinflammatory cytokine. Following targeted Cre-mediated deletion of Ikk in Ikk DeltaHep mice (a strain highly susceptible to chemically-induced hepatotoxicity and hepatocarcinogenesis), CD74 is abundantly expressed by hepatocytes throughout liver acini (as detected by specific Western blots and immunohistochemical stains); it is not observed in either control Ikk F/F hepatocytes or embryonic fibroblasts from Ikk -/- mice. Constitutive CD74 expression in Ikk DeltaHep hepatocytes is also accompanied by significantly augmented expression of CD44 and genes associated with antigen processing and host defense. These observations suggest that Ikk DeltaHep hepatocytes might directly respond to MIF signaling, accounting partly for the enhanced susceptibility of Ikk DeltaHep mice to hepatotoxins and hepatocarcinogens, and also might exhibit unusual immunological properties including antigen presentation. Total RNA samples were obtained from four 6-8 week-old male mice. The four RNA samples were processed as described in the PROTOCOLS section below.

Publication(s) associated with this dataset:
Back
to Top