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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Patent Detail: Method of Detecting DNA Single Strand Breaks

Patent Detail

Superfund Research Program

Method of Detecting DNA Single Strand Breaks

Number: 6,913,878
Year: 2005
Patent Status: Expired
Authors: Nakamura, J., J.A. Swenberg

A method of detecting increased levels of DNA single strand breaks in a eukaryotic cell sample, comprising the steps of: (a) contacting a eukaryotic cell sample to a water-soluble tetrazolium salt under conditions in which said tetrazolium salt is converted to a formazan dye in said cell sample in the presence of NADH or NADPH; and then (b) detecting the presence of the formazan dye in said cell sample, with decreased levels of the formazan dye indicating increased levels of DNA single strand breaks in the eukaryotic cell sample.

Associated Projects:

  • University of North Carolina-Chapel Hill: Biomarkers of Exposure versus Effect: Improving the Scientific Basis for Risk Assessment
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