Project Summary (1995-2000)

This continuing project is directed toward the development of DNA biomarkers and related multi-component predictive models for cancer risk assessment at Superfund sites. An integrated GC-MS/FT-IR analysis model is being developed for fish exposed to environmental pollutants. English sole are being obtained from contaminated and non-contaminated areas and GC-MS/FT-IR analyses is being performed. Integrated GC-MS/FT-IR models form a basis for establishing genotoxic injury and cancer risk at Superfund sites. These data are being compared with data from in vitro experiments with pure substrates under radical-generating conditions. The influence of cellular iron deposits, ferritin status, DNA melting point values and related parameters is being factored in, notably with a view to understanding promotional aspects of carcinogenesis.