Superfund Research Program
Title: Creation of an anti-inflammatory GR cistrome by TLR4 signaling
Accession Number: E-GEOD-31796
Link to Dataset: http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31796/
Repository: ArrayExpress
Data Type(s): Gene Expression
Experiment Type(s): ChIP-seq
Organism(s): Mus musculus
Summary: An unresolved molecular paradox is how the glucocorticoid receptor (GR) activates some genes while potently repressing others. We carried out genome-wide localization and expression profiling experiments in primary bone marrow-derived mouse macrophages treated with Dexamethasone in the presence or absence of LPS. Unexpectedly, we find that the anti-inflammatory GR cistrome, which is principally composed of 'canonical' GREs colocalizing with NFkB and AP-1 co-enriched with the myeloid lineage factors C/EBP and Pu.1, is shaped by TLR4-directed chromatin dynamics, suggesting that context rather than sequence may be a critical determinant of function. Identification of GR, cJun, NFkB(p65) binding sites in primary bone-marrow derived macrophages unstimulated and LPS-stimulated (3hrs) that were untreated or pre-treated with Dexamethasone for 16 hrs
Publication(s) associated with this dataset:- Uhlenhaut N, Barish GD, Yu RT, Downes M, Karunasiri M, Liddle C, Schwalie P, Hubner N, Evans RM. 2013. Insights into negative regulation by the glucocorticoid receptor from genome-wide profiling of inflammatory cistromes. Mol Cell 49(1):158-171. doi:10.1016/j.molcel.2012.10.013 PMID:23159735 PMCID:PMC3640846
- University of California-San Diego: Toxicogenomic Analysis of Nuclear Xenobiotic Receptors