Title: Comparisons between liver tissues and freshly isolated hepatocytes from Ikk F/F and Ikk Dhep (Ikk -deleted) mice

Accession Number: E-GEOD-15476

Repository: ArrayExpress

Data Type(s): Gene Expression

Experiment Type(s): Transcription profiling by array

Organism(s): Mus musculus

Summary: CD74, a Type II membrane glycoprotein and MHC class II chaperone (Ii), is normally expressed by cells associated with the immune system. CD74 also forms heterodimers with CD44 to generate receptors to macrophage migration inhibitory factor (MIF), a proinflammatory cytokine. Following targeted Cre-mediated deletion of Ikk in Ikk DeltaHep mice (a strain highly susceptible to chemically-induced hepatotoxicity and hepatocarcinogenesis), CD74 is abundantly expressed by hepatocytes throughout liver acini (as detected by specific Western blots and immunohistochemical stains); it is not observed in either control Ikk F/F hepatocytes or embryonic fibroblasts from Ikk -/- mice. Constitutive CD74 expression in Ikk DeltaHep hepatocytes is also accompanied by significantly augmented expression of CD44 and genes associated with antigen processing and host defense. These observations suggest that Ikk DeltaHep hepatocytes might directly respond to MIF signaling, accounting partly for the enhanced susceptibility of Ikk DeltaHep mice to hepatotoxins and hepatocarcinogens, and also might exhibit unusual immunological properties including antigen presentation. Total RNA samples were obtained from four 6-8 week-old male mice. The four RNA samples were processed as described in the PROTOCOLS section below.

Publication(s) associated with this dataset:

• Koch KS, Maeda S, Karin M, Leffert HL, He G. 2009. Targeted deletion of hepatocyte Ikkbeta confers growth advantages. Biochem Biophys Res Commun 380(2):349-354. doi:10.1016/j.bbrc.2009.01.085 PMID:19171122 PMCID:PMC2663043

Project(s) associated with this dataset:

• University of California-San Diego: Effects of Superfund Toxicants on Liver Cancer Progenitor Cells