Title: Global transcriptome changes in HepG2 cells exposed to copper

Accession Number: GSE9539

Link to Dataset: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE9539

Repository: Gene Expression Omnibus (GEO)

Data Type(s): Gene Expression

Experiment Type(s): Expression profiling by array

Organism(s): Homo sapiens

Summary: Our hypothesis is that copper modulates the activity of multiple intracellular signal transduction pathways to affect transcription. We have previously shown that copper activates transcription through both metal- and oxidative stress-responsive signal transduction pathways. Since the global molecular mechanisms underlying copper toxicity have not been well elucidated in humans, we have profiled transcriptome changes in HepG2 cells exposed to 100, 200, 400 and 600 uM copper for 4, 8, 12 and 24 hours using a human oligonucleotide microarray. Differentially expressed genes were identified, and integrated into biological and functional pathways through Gene Ontology analysis. Global gene expression profile was overlaid onto biomolecular interaction networks and signal transduction cascades using pathway mapping and interactome identification.

Publication(s) associated with this dataset:

• Song MO, Li J, Freedman JH. 2009. Physiological and toxicological transcriptome changes in HepG2 cells exposed to copper. Physiol Genomics 38(3):386-401. doi:10.1152/physiolgenomics.00083.2009 PMID:19549813 PMCID:PMC3774564

Project(s) associated with this dataset:

• Duke University: Mechanism of Environmental Stress-Induced Developmental Abnormalities