Superfund Research Program
Title: Functional profiling in yeast with zinc-limitation
Accession Number: GSE37254
Link to Dataset: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE37254
Repository: Gene Expression Omnibus (GEO)
Data Type(s): Gene Expression
Organism(s): Saccharomyces cerevisiae
Summary: Zinc is an essential nutrient because it is a required cofactor for many enzymes and transcription factors. To discover genes and processes in yeast that are required for growth when zinc is limiting, we used genome-wide functional profiling. As a result, we identified over 400 different genes required for optimal growth under zinc-limiting conditions. Among these were several targets of the Zap1 zinc-responsive transcription factor. Their importance is consistent with their up-regulation by Zap1 in low zinc. We also identified genes that implicate Zap1-independent processes as important. These include endoplasmic reticulum function, oxidative stress resistance, vesicular trafficking, peroxisome biogenesis, and chromatin modification. Our studies also indicated the critical role of macroautophagy in low zinc growth. Finally, as a result of our analysis, we discovered a previously unknown role for the ICE2 gene in maintaining ER zinc homeostasis.
Publication(s) associated with this dataset:- North M, Steffen J, Loguinov AV, Zimmerman GR, Vulpe CD, Eide DJ. 2012. Genome-wide functional profiling identifies genes and processes important for zinc-limited growth of Saccharomyces Cerevisiae. PLoS Genet 8(6):e1002699. doi:10.1371/journal.pgen.1002699 PMID:22685415 PMCID:PMC3369956
- University of California-Berkeley: Genetic Susceptibility to Superfund Chemicals