Title: Characterization of four TCE-dechlorinating microbial enrichments grown with different cobalamin stress and methanogenic conditions

Accession Number: GSE33530

Link to Dataset: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33530

Repository: Gene Expression Omnibus (GEO)

Data Type(s): Gene Expression

Experiment Type(s): Genome variation profiling by array

Organism(s): Dehalococcoides

Summary: Four stable and robust TCE-dechlorinating microbial communities were enriched from TCE-contaminated groundwater under four different conditions exploring two parameters, high and low methanogenic activity (Meth and NoMeth), with and without vitamin B12 supplement (MethB12 and NoMethB12, Meth and NoMeth, respectively). Identical amounts of lactate (2.7 mmol) and TCE (20 μl) were supplied as electron donor and electron acceptor. All four cultures were capable of reductively dechlorinating TCE to VC and ethene. Genomic DNA of the four enrichments was applied on a quad-Dhc-genome microarray in order to characterize the gene content of Dehalococcoides species present in the four enrichments

Publication(s) associated with this dataset:

• Men Y, Lee PH, Harding KC, Alvarez-Cohen L. 2013. Characterization of four TCE-dechlorinating microbial enrichments grown with different cobalamin stress and methanogenic conditions. Appl Microbiol Biotechnol 97(14):6439-6450. doi:10.1007/s00253-013-4896-8 PMID:23640361

Project(s) associated with this dataset:

• University of California-Berkeley: Meta-Omics of Microbial Communities Involved in Bioremediation