Title: Effects of arsenic and Pseudomonas aeruginosa infection on gene expression in human airway epithelial cells

Accession Number: GSE97036

Link to Dataset: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE97036

Repository: Gene Expression Omnibus (GEO)

Data Type(s): Gene Expression

Experiment Type(s): Expression profiling by high throughput sequencing

Organism(s): Homo sapiens

Summary: Purpose: To determine effects of arsenic on gene expression in polarized primary human bronchial epithelial (HBE) cells and impact on transcriptional response to Pseudomonas aeruginosa infection Methods: mRNA profiles of HBE cells from 6 donors exposed to 0, 5, 10 or 50 ug/L total arsenic +/- Pseudomonas aeruginosa (48 samples) were generated using Illumina sequencing, aligned in CLC Genomics workbench and analyzed for DE in EdgeR Findings: 20-30 million reads were mapped per sample and transcripts were identified that were significantly differentially expressed in response to arsenic and Pseudomonas aeruginosa

Publication(s) associated with this dataset:

• Goodale BC, Rayack EJ, Stanton BA. 2017. Arsenic alters transcriptional responses to Pseudomonas aeruginosa infection and decreases antimicrobial defense of human airway epithelial cells. Toxicol Appl Pharmacol 331:154-163. doi:10.1016/j.taap.2017.06.010 PMID:28625800 PMCID:PMC5568502

Project(s) associated with this dataset:

• Dartmouth College: Arsenic and Innate Immunity in Human Lung