Superfund Research Program
Methods for Detection of Single Strand Breaks in DNA
Patent Status: Pending
Authors: Luke, A.M., J. Nakamura, J.A. Swenberg
Methods are provided for detecting single strand breaks in DNA sample comprising: contacting the DNA with a neutral hydroxylamine, denaturing the DNA and detecting single strand breaks in the denatured DNA. Methods are also provided for the detecting single strand breaks in DNA from a cell, the methods comprising lysing the cell, denaturing the DNA from the lysed cell, wherein the DNA is contacted with a neutral hydroxylamine prior to and/or during the denaturing step and detecting single strand breaks in the DNA from the cell. In addition, methods are provided for distinguishing between true single strand breaks and artifactual single strand breaks resulting from cleaved alkaline labile sites.
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