Project Summary (1995-2000)

The Genotoxicity project tests extracts from wood-preserving and oily waste sites for the induction of bulky/aromatic DNA adducts and oxidative DNA damage. Two novel ³²P-postlabeling genotoxicity assays previously developed with support by the Superfund grant are employed for these determinations. The first test is a short-term mouse bioassay, while the second is an in vitro assay without metabolic activation. The samples are obtained from Project 4. In addition to the two tests, another ³²P-postlabeling-based assay, which determines the effects of waste site toxicants, particularly tumor promoters, on hepatic type I I-compounds, is combined with the mouse assay. Mechanism-oriented experiments focus on possible interactions of components of the complex chemical mixtures from hazardous waste sites resulting in altered genotoxicity, relationships between tumor-initiating and genotoxic activity of waste site extracts, and various factors determining the formation of DNA adducts in vitro by aqueous waste site extracts.