Superfund Research Program
Development and Applications of Integrated Cell-Based Bioassays
Project Leader: Michael S. Denison
Grant Number: P42ES004699
Funding Period: 1995-2015
Project Summary (1995-2000)
The overall goal of this project is to develop biologically- based detection assays for environmental agents based on the existing knowledge of their mechanisms of action. This project utilizes three approaches towards this end. In the first, the ability of certain agents to stimulate gene expression by binding to and activating chemical-specific DNA transcription factors is monitored. Secondly, the ability of environmental toxicants to alter biochemical processes involved in the regulation of cell growth and differentiation is being characterized. Finally, cell stress is being monitored through the assessment of heat shock protein isoform expression and subcelluar localization.