Superfund Research Program
Pulmonary Response to Toxicants in Susceptible Populations: Alterations Following In Utero and Early Postnatal Exposure
Project Leader: Robert Clark Lantz
Co-Investigator: Scott Boitano
Grant Number: P42ES004940
Funding Period: 2005-2020
Project Summary (2010-2015)
Growth and development requires the temporal and spatial coordinated expression of genes and gene products. During this critical time, in utero and early postnatal exposure to toxicants has the potential to affect gene expression, altering organ structure and physiological function. However, only limited attention has been paid to the effects of environmentally relevant exposures to toxicants during these critical periods of development.
Inorganic arsenic is a ubiquitous environmental toxicant, found in high concentrations throughout the world. Drinking water exposures to high levels of arsenic either in utero or during early childhood development led to an increased risk of dying from lung cancers and chronic lung disease in adults. Dr. Lantz's in animal models has demonstrated that following in utero and early postnatal exposure to arsenic, airway response to methacholine was increased in a dose dependent manner. This change appears to be permanent and the response is specific for the early developmental exposure. While exposures from ingestion of arsenic can lead to alterations, the inhalation route of exposure is also relevant to the lung. In utero and/or postnatal exposure to cigarette smoke, ambient urban air particles or metals leads to increased airway reactivity, decreased surface to volume ratios in the lung and altered lung function in the offspring. Therefore, the researchers hypothesize that inhalation of dusts containing arsenic during sensitive developmental times will result in altered pulmonary function and structure in adults. The evaluation of the direct effects of inhaled arsenic and the potential interactions of inhaled arsenic and ingested arsenic are the emphasis of this project. The researchers are evaluating four aims:
- Aim 1 will examine the effects of inhalation of arsenic and arsenic containing particles to pregnant mice (in utero exposure);
- Aim 2 will examine the effects of early postnatal exposures to these compounds; and
- Aim 3 will examine the effect of combined in utero and postnatal exposures.
- Aim 4 will evaluate the effect of inhalation in combination with ingestion of arsenic.